บทคัดย่องานวิจัย

Phytochemical mediation of post-harvest insect resistance in tropical maize

Burt, Andrew J

M.Sc., University of Ottawa (Canada), 2003, 141 pages

2003

บทคัดย่อ

Phytochemical mediation of post-harvest insect resistance in tropical maize

The resistance of maize grain ( Zea mays L.) to the stored insect pest, Sitophilus zeamais , was investigated in relation to the grain content of two groups of phytochemicals. The distribution of hydroxycinnamic acid amide conjugates (HAACs) in Mexican landraces was investigated and the toxicity of the HAACs in maize grain versus S. zeamais was examined by short-term feeding bioassays and a two-generation life cycle. Results suggest that the HAACs are not an effective defence compound against S. zeamais , but may be effective against generalist feeders.

The function of cell wall bound phenylpropanoids to S. zeamais resistance in a collection of maize grain with variable post-harvest resistance and in a Quantitive Trait Locus (QTL) mapping population was examined. Correlational data suggest that diferulic acid content is an important resistance factor. Evidence was found for nine chromosomal locations where QTLs for cell wall bound phenylpropanoid content correspond with QTLs for insect resistance. These results indicate a defence against grain attack by S. zeamais mediated by cell wall bound phenylpropanoids.