Characterization of yeast antagonistic towards citrus green mould by electrophoretic karyotyping
G. Arras
ISHS Acta Horticulturae 599: 617-621.
2003
บทคัดย่อ
The inhibitory activity shown by different antagonist yeasts isolated from fig, prickly pear and citrus fruits and leaves against Penicillium digitatum, causal agent of citrus green mould was investigated. The results show that, among 237 strains isolated, the most effective microorganisms belong to the species Pichia guilliermondii and Candida famata. In particular, isolates 5A of P. guilliermondii and 43E of C. famata (108 cells ml-1) showed inhibition values ranging between 98 and 100% against P. digitatum (105 and 106 conidia ml-1) on artificially wounded orange and satsuma fruits. The least effective yeasts belong to the species Rhodotorula mucillaginosa, R. minuta and Zygoascus hellenicus. Pulsed-field gel electrophoresis (PFGE) was used to find difference in the electrophoretic karyotype and to obtain markers useful for isolate identification.