บทคัดย่องานวิจัย

Postharvest control of established infection of Penicillium digitatum on lemons by combining the biocontrol agent Pantoea agglomerans CPA-2 and a curing treatment

Usall J.; Plazza P.; Smilanick J.L.; Teixido N.; Abadias M. and Vinas, I.

5th International Postharvest Symposium . Volume of Abstract . Verona, Italy 6-11 June 2004, p.104

2004

บทคัดย่อ

Postharvest control of established infection of Penicillium digitatum on lemons by combining the biocontrol agent Pantoea agglomerans CPA-2 and a curing treatment  Biological control using microbial antagonists has gained considerable attention as a promising alternative to chemicals. Studies with Pantoea agglomerans CPA-2 have demonstrated its efficacy to control green and blue mold o­n oranges and o­n mandarins. Recent studies carried out in our laboratory had demonstrated that a curing treatment at 33°C for 65 h controlled effectively green and blue mould o­n oranges and lemons stored under ambient conditions.

In this work, the effectiveness of the stain P. agglomerans CPA-2 alone or combination witha curing treatment at 33°C for 65 h to control green mould was evaluated o­n lemons stored at ambient and cold storage. Furthermore, the efficacy of this biocontrol agent to control established infections of Penicillium digitatum in wounded-inoculated lemons was determined. Our results have showed that an application of P. agglomerans CPA-2 at 2 108 cfu ml-1 effectively reduced green mould incidence o­n recently inoculated lemons stored at different temperatures from 5°C to 25°C. However, it failed to control established infections of green mould of more than 24h. Combining the biocontrol agents and curing treatment at 33°C for 65 h controlled 24-h old infections o­n artificially inoculated lemons stored at 20°C for 14 days and o­n naturally infected lemons stored at 10°C for 3 weeks plus 7 days at 20°C (shelf-life period).

The improvement obtained combining P. agglomerans and the curing treatment compared to each treatment alone indicates a synergistic effect between them. Population dynamics of P. agglomerans in wound fruits were evaluated to know the effect of the application of curing treatment before or after biocontrol agent applications. P.