ศูนย์นวัตกรรมเทคโนโลยีหลังการเก็บเกี่ยว

Characterization of the ethylene receptor gene homologues from apple fruit.

Tatsuki M., Endou A.

5th International Postharvest Symposium . Volume of Abstract . Verona, Italy 6-11 June 2004, p.79

2004

บทคัดย่อ

Ethylene plays an important role in the initiation and continuation of ripening in all climacteric fruits, including apple.Ethylene perception and signal transduction have been studied at the genetic and biochemical levels in Arabidopsis and other species.Studies of ethylene perception have shown that ethylene receptors negatively regulate the ethylene response.To elucidate the role of the ethylene receptors during apple ripening and storage, we isolated cDNAs of three putative ethylene receptor genes by RT-PCR using degenerate primers from ripening apple fruit.The nucleotide sequences of two cDNA clones were identical to that of Md-ETR1 and Md-ERS1 that were previously reported.The nucleotide sequence of another cDNA clone is similar to that of Md-ERS1, which lacked a response regulatory domain at its C-terminus.Thus, the cDNA was named Md-ERS2.We examined their expression patterns in two apple varieties, ‘Orin’ and ‘Fuji’, which have distinct characteristics in ethylene production and storage life.Although Md-ETR1 appeared to be constitutively expressed in two apple varieties, Md-ERS1 and Md-ERS2 expressed o­nly at the climacteric fruit stage.In both apple varieties, treatment with 1- methylcyclopropene (1-MCP), a highly potent inhibitor of ethylene action, suppressed ethylene production and decreased expressions of three receptor genes.Md-ETR mRNA declined from 7 days after 1-MCP treatment but Md-ERS1 and Md-ERS2 mRNAs rapidly decreased within 1 day after 1-MCP treatment.Ethylene receptor mRNAs accumulated more abundantly in ‘Fuji’, which has long storage life, than in ‘Orin’.This result supports the model that ethylene receptors negatively regulate the ethylene response in apple.