บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Different response of apple and peach fruits to 1-MCP. A case of different sensitivity to ethylene?

Dal Cin V., Rizzini F.M., Botton A., Ziliotto F., Danesin M., Tonutti P.

5th International Postharvest Symposium . Volume of Abstract . Verona, Italy 6-11 June 2004, p.77

2004

บทคัดย่อ

Different response of apple and peach fruits to 1-MCP. A case of different sensitivity to ethylene?  1-MCP is known as an antagonist of ethylene action.However, not all climacteric fruits respond similarly to 1-MCP treatment.When applied at apples 1-MCP is particularly effective in delaying ripening, while in peaches it seems to slow down the rate of softening o­nly during the incubation period but a quick recovery of ripening parameters occurs in the post-treatment phase.In order to elucidate the different behaviour, specific trials o­n peaches (cv. Summer rich treated with 1-MCP up to 1 ppm for different times) and apples (cv. Gala and G. delicious treated with 1-MCP at 0.625 ppm for 24 h) have been performed.

As expected, apple fruits responded to 1-MCP remaining firm and producing little ethylene for several days at 20 °C, whereas in peach fruits firmness quickly decreased and ethylene evolution dramatically increased after few hours from the end of the treatments.A molecular approach was used to investigate the sensitivity of the fruits to ethylene.In particular, ethylene receptors and CTR1-like gene expression was monitored all over the experiment for both fruits via TR-PCR.Md-ACO1 and Pp-ACO1 expression was studied as indicators of the modulation of ethylene biosynthetic pathway.Pp-ACO1 and Pp-ERS1 expression was decreased by 1-MCP treatment but sharply increased just few hours after the end of the treatment.Furthermore, expression of some of the genes encoding enzymes both ethylene up-regulated and involved in fruit softness was studied.

Polygalacturonase and Pectate lyase gene expression in peaches dramatically decreased after treatment but considerably increased just after few hours concomitantly with the increase of Pp-ERS1 expression.The relation between ethylene receptors and cell wall hydrolase transcripts is reported as a tool to describe differences in sensitivity to 1-MCP and ripening physiology in apples and peaches.