บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Fate of [14C]diphenylamine in stored apples.

Heasook KimKang; Robinson, R. A.; Wu Jinn;

Journal of Agricultural and Food Chemistry Year: 1998 Vol: 46 Issue: 2 Pages: 707-717 Ref: 9 ref.

1998

บทคัดย่อ

Fate of [14C]diphenylamine in stored apples.

The nature and magnitude of residues in Red Delicious apples treated with [14C]diphenylamine (DPA; used to control apple scald) was investigated following treatment and storage at reduced temperature (0 plus or minus 2 deg C). Additional apples were treated with a mixture of [14C]DPA and deuterium-labelled DPA (D-10-DPA) for use in metabolite identification. Stored apples were sampled at 16 different intervals throughout a 40-week period. Total radioactive residue levels remained constant over the entire testing interval. The parent chemical and several metabolites were identified on the final harvest peel and pulp samples by quantitative and qualitative analytical methods. Most of the terminal residue after 40 weeks, which was confined largely in the peel, consisted of unmetabolized DPA. Residues in pulp, however, were largely composed of glycosyl conjugates of several hydroxylated diphenylamine (OH-DPA) metabolites. The major polar metabolite identified in stored apples was a glucose conjugate

 of 4-hydroxydiphenylamine (4-OH-DPA). Additional metabolites, characterized as glycosyl conjugates of 2-OH-DPA, 3-OH-DPA, 4-OH-DPA, or dihydroxy-DPA, were also detected along with their intact (i.e., nonconjugated) forms in apple pulp.