บทคัดย่องานวิจัย

Biocontrol by yeasts of blue mould of citrus fruits and the mode of action of an isolate of Pichia guilliermondii.

Arras, G.; Cicco, V. D.; Arru, S.; Lima, G.;

Journal of Horticultural Science and Biotechnology Year: 1998 Vol: 73 Issue: 3 Pages: 413-418 Ref: 15 ref.

1998

บทคัดย่อ

Biocontrol by yeasts of blue mould of citrus fruits and the mode of action of an isolate of Pichia guilliermondii.

Nineteen yeast isolates from different fruits were investigated for antagonistic activity against blue mould (Penicillium italicum) on artificially wounded citrus fruits. Strains 1A, 5A and 29A of P. guilliermondii, strains 21D, 22D and 30F of Candida famata, and strain 13L of C. sake reduced infection compared with the controls. P. guilliermondii 5A was the most effective antagonist, and its mode of action was investigated. Its activity against P. italicum decreased when the number of yeast cells inoculated in the wound was reduced. It showed good ability to colonize the wound site and grow rapidly, and cell numbers increased by up to 60-fold within 24 h. SEM of the wound showed attachment of the yeast cells to P. italicum hyphae. The high antagonistic activity of isolate 5A appeared to be related to its capability to occupy space and use nutrients available in the wound, thus depriving P. italicum of them. 5A also induced the biosynthesis of the phytoalexins scoparone and scopoletin in the wou

nd, although this did not appear to have an essential role in biocontrol of the pathogen.