Biocontrol of mold growth in high-moisture wheat stored under airtight conditions by Pichia anomala, Pichia guilliermondii, and Saccharomyces cerevisiae.
Petersson, S.; Schnurer, J.;
Applied and Environmental Microbiology Year: 1995 Vol: 61 Issue: 3 Pages: 1027-1032 Ref: 20 ref.
1995
บทคัดย่อ
The abilities of Pichia anomala, Pichia guilliermondii, and Saccharomyces cerevisiae to inhibit the growth of the mould Penicillium roqueforti in nonsterile high-moisture wheat were compared by adding 103 Penicillium roqueforti spores and different amounts of yeast cells per gram of wheat (packed in glass tubes and incubated at 25 deg C). Pichia anomala reduced growth on agar plates for all of the mould species tested in a dose-dependent manner. Aspergillus fumigatus and Eurotium amstelodami were the most sensitive, while Penicillium italicum and Penicillium digitatum were the most resistant. Pichia anomala had the strongest antagonistic activity in wheat, with 105 and 106 c.f.u./g completely inhibiting the growth of Penicillium roqueforti. Inhibition was least pronounced at the optimum temperature (21 deg C) and water activity (0.95) for the growth of Penicillium roqueforti. Pichia guilliermondii slightly reduced the growth of Penicillium roqueforti in wheat inoculated with 105 and 106 yeast c.
f.u./g. Saccharomyces cerevisiae inhibited mould growth only weakly at the highest inoculum level. Pichia anomala grew from 103 to 107 c.f.u./g of wheat in 1 week. To reach the same level, Pichia guilliermondii had to be inoculated at 104 c.f.u. while Saccharomyces cerevisiae required an inoculum of 105 c.f.u. to reach 107 c.f.u./g of wheat.