บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Post-harvest regulation of sucrose accumulation in transgenic potatoes: role and properties of potato tuber UDP-glucose pyrophosphorylase.

Sowokinos, J.;

The molecular and cellular biology of the potato. Year: 1994 Issue: Ed. 2 Pages: 81-106 Ref: 66 ref.

1994

บทคัดย่อ

Post-harvest regulation of sucrose accumulation in transgenic potatoes: role and properties of potato tuber UDP-glucose pyrophosphorylase.

ABSTRACT :

 

This chapter describes the properties and role of UTP-glucose-1-phosphate uridylyltransferase (UGPase; EC 2.7.7.9) in catalysing the first reaction unique to the sucroneogenic pathway via the formation of UDP-glucose. The importance of UGPase to potato growth and development is briefly reviewed and data are presented which show a coordinated expression of UGPase RNA with RNA of several other enzymes involved in the conversion of sucrose to starch in developing tubers. Initial results from transgenic potato tubers (cv. Desiree) expressing sense and antisense UGPase constructs are discussed in relation to postharvest sucrose content resulting from storage at 4 deg and 12 deg C. Estimates of metabolic control coefficients (C values) relating UGPase activity to sucrose accumulation, from initial short-term experiments (6 weeks), suggest that UGPase can partially control sucroneogenesis in stored potato tubers.