บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Postharvest evaluation of transgenic tomatoes with reduced levels of polygalacturonase: processing, firmness and disease resistance.

Kramer, M.; Sanders, R.; Bolkan, H.; Waters, C.; Sheehy, R. E.; Hiatt, W. R.;

Postharvest Biology and Technology Year: 1992 Vol: 1 Issue: 3 Pages: 241-255 Ref: 29 ref.

1992

บทคัดย่อ

Postharvest evaluation of transgenic tomatoes with reduced levels of polygalacturonase: processing, firmness and disease resistance.

The pectin-degrading enzyme polygalacturonase (PG) normally accumulates to high levels in ripe tomato fruits. Processing and fresh market tomato genotypes were transformed with an antisense PG construct and lines were developed which produced fruits with PG levels reduced by more than 99%. To determine the effect of reduced PG activity on both processing and fresh market tomato products, transgenic tomato plants were field-tested in 3 separate trials during 1989 and 1990. Analysis of field-grown material demonstrated a significant increase in the serum viscosity of processed juice and paste, and a significant decrease in softening during storage of fresh market fruit relative to non-transgenic controls. Fruits with reduced PG were also shown, in laboratory tests, to have increased resistance to Geotrichum candidum and Rhizopus stolonifer, fungi which normally infect ripening fruits. Thus, manipulation of PG levels results in multiple phenotypic changes which have important bearings on fruit qual

ity through their effects on the pectic substances.