บทคัดย่องานวิจัย

Regulation of fermentative metabolism in avocado fruit under oxygen and carbon dioxide stresses.

D. Ke, E. Yahia, B. Hess, L. Zhou and A.A. Kader

Journal of the American Society for Horticultural Science. Volume 120, Number 3, May 1995. Pages 481-490.

1995

บทคัดย่อ

Regulation of fermentative metabolism in avocado fruit under oxygen and carbon dioxide stresses.

'Hess' avocado (Persea americana Mill.) fruit were kept in air, 0.25% O2 (balance N2), 20% O2 + 80% CO2, or 0.25% O2 + 80% CO2 (balance N2) at 20C for up to 3 days to study the regulation of fermentative metabolism. The 0.25% O2 and 0.25% O + 80% CO2 treatments caused accumulations of acetaldehyde and ethanol and increased NADH concentration, but decreased NAD level. The 20% O2 + 80% CO2 treatment slightly increased acetaldehyde and ethanol concentrations without significant effects on NADH and NAD levels. Lactate accumulated in avocadoes kept in 0.25% O2. The 80% CO2 (added to 0.25% O2) did not increase lactate concentration and negated the 0.25% O2-induced lactate accumulation. Activities of PDC and LDH were slightly enhanced and a new isozyme of ADH was induced by 0.25% O2, 20% O2 + 80% CO2, or 0.25% O2 + 80% CO2; these treatments partly reduced the overall activity of the PDH complex. Fermentative metabolism can be regulated by changes in levels of PDC, ADH, LDH, and PDH enzymes and/or by metabolic control of the functions of these enzymes through changes in pH, ATP, pyruvate, acetaldehyde, NADH, or NAD.