Cold-induced accumulation of 1-aminocyclopropane 1-carboxylate oxidase protein in Granny Smith apples
J-M. Leli?vre, L. Tichit, L. Fillion, C. Larrigaudi?re, M. Vendrell and J-C. Pech
Postharvest Biology and Technology Vol: 5 Issue: 1-2 Pages: 11-17
1995
บทคัดย่อ
Preclimacteric Granny Smith apple (Malus domestica Borkh.) fruits exhibited a sharp increase in ethylene production after 5 days in the cold (4°C), while in the absence of chilling (17°C) ethylene production started to increase only at 35 days. Antibodies raised against a recombinant tomato 1-aminocyclopropane 1-carboxylate oxidase -- the enzyme catalyzing the conversion of ACC into C2H4 -- were used to demonstrate an accumulation of ACC oxidase protein in fruits stored at 4°C which paralleled the increase in ethylene production. Two-dimensional gel electrophoresis indicated that the protein corresponded to one polypeptide species only which was identical in both chilled and non-chilled fruits. These results therefore indicate that ACC oxidase, in addition to ACC synthase, is induced during chilling of pre-climacteric Granny Smith apples and accumulates before any transfer of the fruit to warmer temperatures.