Pichia anomala as a biocontrol agent during storage of high-moisture feed grain under airtight conditions
Stina Petersson, Nils Jonsson and Johan Schnurer
Postharvest Biology and Technology Vol: 15 Issue: 2 Pages: 175-184.
1999
บทคัดย่อ
Pichia anomala is antagonistic against a range of spoilage molds in vitro as well as against Penicillium roqueforti in high-moisture wheat during malfunctioning airtight storage in laboratory experiments. The use of Pichia anomala to improve the postharvest control of Penicillium roqueforti during airtight storage of feed grain was evaluated in outdoor silos. Inoculated and control winter wheat (cultivar Kosack) in 160-kg portions were stored at a water activity of 0.93 for 12 months in silos that were opened twice a week. During the first 2 months, inoculated Pichia anomala increased to about 107 colony-forming units (CFU)/g, while naturally occurring Pichia anomala in the treatments without inoculated yeast increased from 104 to 106 CFU/g. During the same period, CO2 concentrations increased to almost 70% and stabilized at 50¯60%. During the coldest period, O2 concentrations of <1% could be detected between samplings, whereas during the rest of the storage detectable O2 levels were only found immediately after sampling. There were no clear differences in CO2 or O2 levels between treatments. The inoculated Penicillium roqueforti did not grow during the storage period, probably owing to high numbers of Pichia anomala in combination with the high CO2 and low O2 concentrations in the silos. In laboratory experiments, it was found that Pichia anomala survived long-term storage in airtight sealed test tubes better at 15°C than at -20°C. The aerobic stability of moist wheat after 10 and 12 months of silo storage was clearly enhanced by an initial inoculation with Pichia anomala.