Lignin biosynthesis during wound healing of potato tubers in response to gamma irradiation
M. S. Ramamurthy, K. K. Ussuf, P. M. Nair and P. Thomas
Postharvest Biology and Technology Vol: 18 Issue: 3 Pages: 267-272.
2000
บทคัดย่อ
The effect of gamma irradiation on lignin biosynthesis during wound healing
in potato tubers was studied by [U-14C] phenylalanine incorporation
into lignin and monitoring the activities of key enzymes involved in the
lignification process. There was a 40% reduction in lignin biosynthesis during
wound healing in response to gamma irradiation. The level of the first enzyme
involved in lignin biosynthesis, phenylalanine ammonia lyase (PAL), was
five-fold higher in irradiated potatoes than in control tubers during wound
healing. However the level of another key enzyme, cinnamyl alcohol
dehydrogenase (CAD) was found to be 30% less in irradiated potatoes. There was
no significant change in peroxidase activity and its isozyme pattern during
wound healing. Differential regulation of different enzymes of lignin
biosynthesis in response to gamma irradiation may be responsible for the
decreased lignin biosynthesis.