Imbalanced expression of stay-green 1 alleles in banana AAB/ABB cultivars prevents high-temperature-induced green ripening as in AAA Cavendish fruit
Qing Wu, Zhuang-zhuang Ma, Yan-ling Qin, Ying-man Li, Bing-zhi Huang, Xue-lian Zhang, Lina Du, Jun Song, Zhao-qi Zhang and Xue-qun Pang
Postharvest Biology and Technology, Volume 158, December 2019, 110980
2019
บทคัดย่อ
Cavendish banana (Musa AAA) shows green-ripening at high temperatures above 24โ€ฏ°C, while Bluggoe fruit (Musa ABB) turns completely yellow. To understand the genomic constitution of the fruit de-greening trait, we compared fruit ripening and chlorophyll (Chl) degradation in 4 banana cultivars, belonging to the Cavendish (AAA), Mysore (AAB), Pisang Awak (ABB) and Bluggoe (ABB) subgroups, at 30โ€ฏ°C and 20โ€ฏ°C. Compared with fruit at 20โ€ฏ°C, ripening progress of the 4 cultivars at 30โ€ฏ°C, as represented by the change in fruit firmness and expression of fruit ripening-related genes, ACS ACO and PG, was accelerated, while the de-greening of fruits was repressed in the AAA cultivar but enhanced in the 3 hybrids (AAB/ABB). The expression of Stay-Green 1 (SGR1), a key Chl degradation gene, was reduced in the AAA cultivar but induced in the hybrids, correlating to the fruit de-greening patterns. The A- and B-SGR1 alleles, from the A- and B-genome, respectively, and a recombinant of the two alleles (H-SGR1) were identified in the AAB/ABB cultivars. A highly conserved polymorphism code was found to differentiate the A/B/H-SGR1 alleles. Allele-specific expression analysis showed that the Bluggoe fruit transcribed only B-SGR1, while Pisang Awak and Mysore expressed B/H- and A/B-SGR1, respectively, and higher B-SGR1 transcription levels were detected at 30โ€ฏ°C than at 20โ€ฏ°C. Transient expression of A/B/H-SGR1 in tobacco leaves demonstrated that SGR1s were all targeted to chloroplasts, and A-SGR1 showed a weaker Chl degradation capacity than B/H-SGR1. The allelic imbalanced expression (AIE) of SGR1 was demonstrated to confer the different fruit de-greening traits to the AAA and AAB/ABB cultivars at high temperatures.