Inhibitory effect and possible mechanism of carvacrol against Colletotrichum fructicola
Shaopei Pei, Ruiling Liu, Haiyan Gao, Hangjun Chen, Weijie Wu, Xiangjun Fang and Yanchao Han
Postharvest Biology and Technology, Volume 163, May 2020, 111126
2020
บทคัดย่อ
Carvacrol (CVR) is an essential oil and has been reported to exhibit significant inhibitory effects against a wide range of microorganisms. Anthracnose, which is caused by Colletotrichum fructicola is one of the most serious postharvest diseases in pitaya fruit. In this study, we investigate the inhibitory effect of CVR against C. fructicola and elucidate the possible modes of action. The results from our study showed that treatment with CVR could effectively inhibit mycelial growth, spore germination and germ tube elongation of C. fructicola in vitro, significantly reducing the onset and development of anthracnose in red pitaya fruit. Membrane permeability and leakage of cellular components in the hyphae of C. fructicola increased as the concentrations of CVR increased. The results from microscopy indicated that CVR treatment caused abnormalities of hyphal morphology, disruption of cytoplasmic membranes, and disorganization of intracellular organelles. Moreover, CVR treatments induced the generation of reactive oxygen species (ROS), which caused oxidative injury to the cell membrane vialipid peroxidation. The antifungal mechanism of CVR against C. fructicola may be attributed to induced ROS production, increased membrane permeability, abnormalities of ultrastructure, and loss of intracellular contents.