Transcriptome analysis reveals the underlying mechanism of nanocomposite packaging in delaying quality deterioration of Flammulina velutipes
Changzhou Zuo, Qiuhui Hu, Anxiang Su, Fei Pei, Gaoxing Ma, Hui Xu, Minhao Xie, Jianhui Liu, Alfred Mugambi Mariga and Wenjian Yang
Postharvest Biology and Technology, Volume 182, December 2021, 111723
2021
บทคัดย่อ
To expound the molecular mechanism of nanocomposite packaging material (Nano-PM) in delaying quality deterioration of Flammulina velutipes during storage, transcriptome analysis was conducted to observe the changes of gene expression in F. velutipes that was packaged in Nano-PM, polyethylene packaging material (Normal-PM) and no packaging material (No-PM). In comparison with Normal-PM, 379 differentially expressed genes (DEGs) were identified in Nano-PM packaged F. velutipes, comprising of 161 up-regulated genes and 218 down-regulated genes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis indicated that Nano-PM improved the stress resistance of F. velutipes by up-regulating RBOHF and msp2, which were related to reactive oxygen species (ROS) metabolism. Nano-PM also ensured sufficient energy supply by regulating oxidative phosphorylation and glycolysis/gluconeogenesis. Moreover, the regulation of gene expression also delayed programmed cell death (PCD) and lignification. Furthermore, the quantitative real-time PCR (qRT-PCR) results were also in accordance with transcriptome analysis. Therefore, Nano-PM could regulate the expression of specific genes, which in turn alleviated quality deterioration of F. velutipes during storage.