Induction of defense response against Alternaria rot in Zaosu pear fruit by exogenous L-lysine through regulating ROS metabolism and activating defense-related proteins
Yongxiang Liu, Yongcai Li, Yang Bi, Qianqian Jiang, Renyan Mao, Zhitian Liu, Yi Huang, Miao Zhang and Dov B. Prusky
Postharvest Biology and Technology, Volume 179, September 2021, 111567
2021
บทคัดย่อ
L-lysine, an important basic amino acid, its catabolic pathway is activated upon microbial pathogen attack in plants and has a central role in plant immunity. The control effects of exogenous L-lysine treatment on Alternaria rot in postharvest ‘Zaosu’ pear fruit and possible mechanisms involved were studied. The results showed that L-lysine treatment inhibited Alternaria rot development and the effect was negatively correlated with the treatment concentration, lesion diameter of 0.1mM L-lysine treated fruit was 43.7 % lower than that of the control 15 d after treatment. Further studies showed that L-lysine treatment significantly suppressed O2−, H2O2, Malondialdehyde (MDA) content, and enhanced catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) activities and gene expression in pear fruit. β-1,3-glucanase (GLU), and chitinase (CHI) activities and PbrGLU, PbrCHI, and PbrPR-1 gene expression were also induced by exogenous L-lysine. The findings suggest that postharvest L-lysine treatment effectively enhanced disease resistance through inhibiting reactive oxygen species (ROS) production and activating defense-related protein in pear fruit.