Determination of the change of flavonoid components as the defence materials of Citrus unshiu Marc. fruit peel against Penicillium digitatum by liquid chromatography coupled with tandem mass spectrometry
Hae Gyeong Kim, Gon-Sup Kim, Jung Han Lee, Semin Park, Won Young Jeong,Yun-Hi Kim, Jae Hoon Kim, Soo Taek Kim, Young Ah Cho, Won Sup Lee, Soo Jung Lee, Jong Sung Jin, Sung Chul Shin
Food Chemistry, Volume 128, Issue 1, 1 September 2011, Pages 49–54
2011
บทคัดย่อ
A healthy fruit peel of Citrus unshiu Marc. and one infected by Penicillium digitatum were analysed for flavonoids via high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC–MS/MS) in the positive mode with selected ion monitoring (SIM). Among 16 flavonoid components characterised in C. unshiu Marc., four flavanones and nine flavones were identified for the first time. The identified compounds were quantified by HPLC–UV. To investigate the function of the flavonoids as defence materials, the flavonoid content change of the fruit peel inoculated with P. digitatum was monitored by HPLC. The flavonoid concentration in the infected fruit peel decreased initially after the infection and then gradually increased before finally progressively decreasing.