Expression analysis of genes for ethylene biosynthesis enzyme, ethylene perception and signal transduction pathway after pretreatment with ethylene receptor inhibitors of miniature potted roses (Rosa hybrida L.)
Mantana Buanong, Heiko Mibus, Edward C. Sisler and Margrethe Serek
Abstract of 5th National Conference on Postharvest Technology, Miracle Grand Convention Hotel, Bangkok, 28-29 June 2007. 151 p.
2007
บทคัดย่อ
In order to investigate transcript abundance of genes for ethylene biosynthesis enzyme (RhACS1-5), ethylene perception (RhETR1-4) and signal transduction pathway (RhCTR1-2, RhEIN3, RhEIL), expression of these transcripts in petal tissues was examined in two rose cultivars ‘Vanilla’ and ‘Lavender’ during continuous exposure to exogenous ethylene and ethylene-free air after pretreatment with inhibitors of ethylene receptors, 1-MCP, 1-OCP and 1-DCP. Additionally, one of the ethylene-responsive element-binding factor (ERFs) genes was isolated from miniature potted roses (RhERF1) which was 92 bp long, encoding a predicted polypeptide for an Ap2 DNA binding domain and sharing high homology with other reported ERF domains. Northern blot hybridization analysis was used to compare with results of RT-PCR. However, it was difficult to distinguish the hybridization signal of ethylene receptors between RhETR1 and RhETR2, which were highly homologue. RT-PCR analysis revealed that ethylene induced the expression of all genes investigated in control ‘Vanilla’ and ‘Lavender’ petals except RhACS1-2 and RhETR4, respectively, while pretreatments of 1-MCP and 1-OCP led to the suppression of all investigated mRNAs in the presence or absence of ethylene. However, strong expression of RhETR3, RhEIN3 and RhEIL transcripts was detectable in ‘Lavender’ petals pretreated with 1-DCP.