Rapid detection of pepper mild mottle virus in seeds of pepper (Capsicum annuum) by indirect dot-immunobinding assay
X. Li.
Journal of Plant Pathology Volume 90 (2, Supplement) August 2008, Book of Abstract, 9th International Congress of Plant Pathology, August 24-29, 2008 Torino,Italy,. 507 pages.
2008
บทคัดย่อ
Rapid detection of pepper mild mottle virus in seeds of pepper (Capsicum annuum) by indirect dot-immunobinding assay
Pepper mild mottle virus(PMMoV) in pepper seeds was detected by indirect dot-immunobinding assay (I-DIBA) on nitrocellulose membrances and by DAS-ELISA. We found that in PMMoV-positive pepper seed, the detection titre was 1:12800 (w/v) by I-DIBA, and 1:51200 (w/v) by DAS-ELISA. PMMoV in pepper leaf could be detected at maximum dilution of 1:3200 (w/v) by both I-DIBA and DAS-ELISA. Healthy pepper leaf did not give a positive reaction when diluted to 1:800 (w/v). Detection PMMoV in pepper seed by I-DIBA was repeatable and uniform.To clarify rates of PMMoV infection in pepper seeds, seeds of 17 commercial pepper varieties were tested by I-DIBA. In 12 of the varieties, PMMoV was seedborne with different rates from 1.25% to 100%.