New procedure for fumonisins B1 and B2 in corn and corn products by ion pair chromatography with 0-phthaldialdehyde postcolumn derivatization and fluorometric detection.
MIYAHARA, M., AKIYAMA, H., TOYODA, M. and SAITO, Y.
Journal of Agricultural and Food Chemistry 44 : 842-847.
1996
บทคัดย่อ
An analytical method for FB1 and B2 in corn and related foods utilising ion pair chromatography with a postcolumn reaction technique is described. Samples were prepared and purified by tandem solid phase extraction columns (SAX and C18). The fumonisins were derivatised with 0-phthaldialdehyde and N-acetyl-L-cysteine in an in-line reaction coil. Ion pair LC with fluorescence detection was used for the separation and detection. Absolute recoveries of fumonisins ranged from 54 to 110% at 0.04 and 0.08mg/kg, respectively. The detection limit was 20ng. The method is relatively simple and provides a screening method for fumonisins in corn and related foods.