A colorimetric technique for detecting trichothecenes and assessing relative potencies.
Engler, K.H., Coker, R.D. and Evans, I.H.
Applied and Environmental Microbiology 65 : 1854-1857.
1999
บทคัดย่อ
A novel colorimetric toxicity test, based on the inhibition of beta-galactosidase activity in Kluyveromyces marxianus, was tested for a range of mycotoxins. The order of toxicity established with this bioassay was verrucarin A > roridin A > T-2 toxin > diacetoxyscirpenol > HT-2 toxin > acetyl T-2 toxin > neosolaniol > fusarenon X > T-2 triol > scirpentriol > NIV > DON > T-2 tetraol. Verrucarin A had a 50% effective concentration of 2 mg/L. Other mycotoxins including cyclopiazonic acid, FB1, OA, patulin, sterigmatocystin, tenuazonic acid and ZEA, could not be detected at up to 10 mg/L.