บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Detection and quantification of Fusarium culmorum and Fusarium graminearum in cereals using PCR assays.

NICHOLSON, P. SIMPSON, D.R., WESTON, G., REZANOOR, H.N. LEES A.K., PARRY, D.W. and JOYCE, D.

Physiological and Motecular Plant Pathology 53: 17 – 37.

1998

บทคัดย่อ

Detection and quantification of Fusarium culmorum and Fusarium graminearum in cereals using PCR assays.

Random amplified polymorphic DNA assays were used to identify amplification prodicts characteristic of either Fusarium clumorum or F. graminearum. Selected fragments were cloned, sequenced and primer pairs were developed wihich permitted specific detection of F.culmorum or F.graminearum using conventional PCR. Quantitative assays were developed for both F. culmorum and F. graminearum using competitive PCR assay was used to study the colonisation of wheat grain by trichothecene producting and non – producing isolates of F.graminearum. Colonisation of grain by trichothecene producing isolates was greater than that by non – producing isolates, supporting the view that trichothecenes act as vinilence factors in the colonisation of wheat by F.grminearum.