Robotic automated clean – up for detection of fumonisins B1 and B2 in corn and corn – based feed by high – performance liquid chromatography.
Dilkin, P., Mallmann, C.A., Dealmeida, C.A.A. and Correa, B.
Journal of Chromatography A 925: 151 – 157.
2001
บทคัดย่อ
An HPLC system with fluorescence detection and an automated on – line solid – phase extraction procedure for FB1 and FB2 in corn and corn – based products is described. The best methodology was found to be extraction with acetonitrile – water and cleanup on C18 end capped disposable extraction cartridges. The system has the advantage of running in an unattended mode of operation and allows processing of 40 samples without system refuel, performing cleanup, o- phthaldialdehyde derivatisation, injection and fumonisin detection by fluorescence detection linked to a computer integrator for automated data processing. Average recoveries for corn and corn – based feed were 92.6 and 88.3%, respectively, with relative standard deviations (RSDs) of 5.04 and 6.22% for FB1, and 91.2 and 89.0% with RSDs of 5.84 and 7.88% for FB2, Detection limits for corn and corn – based feed were approximately 0.03 and 0.05 mg/kg for FB1 and FB2, respectively.