Genotoxicity testing of extracts from aflatoxin - contaminated peanut meal, following chemical decontamination.
Hoogenboom, L.A.P., Polman, T.H.G., Neal, G.E., Verma, A., Guyomard, C., Tulliea, J., Gautier, J.P., Coker, R.D., Nagler, M.J., Heidenreich, E. and De
Food Additives and Contaminants 18: 329 – 341.
2001
บทคัดย่อ
A new method based on the use of florisil and C18 solid phase extraction columns has been developed for the preparation of extracts from aflatoxin – contaminated peanut meal which has been decontaminated by a small – scale version of an industrial decontamination process based on ammoniation. The method allowed testing with in vitro genotoxicity assays without interference of the residual AFB1. Recovery of degradation products in the extracts was evaluated by the use of [14C] AFB1 added to naturally – contaminated peanut meal. Following decontamination, more than 90% of the label could not be extracted from the meal indicating a total AFB1 reduction of more than 99%. The extraction procedure resulted in AFB1 – rich and AFB1 – poor fractions, the later containing half of the extractable decontamination products but less than 1% of the residual AFB1. Testing in the Salmonella/microsome mutagenicity assay of the original crude extracts and AFB1 – rich fractions prepared from non – treated and decontaminated mea, showed the positive results expected from the AFB1 contents as determined by HPLC analysis, Analysis and tesing of the AFB1 – poor fractions showed that the various decontamination processes not only resulted in a successful degradation of AFB1 but also did not produce other potent mutagenic compounds.