Determination of trichothecenes in wheat by capillary gas chromatography with flame ionisation detection.
SCHOTHORST, R.C. and JEKEL, A.A.
Food Chemistry 73: 111 – 117.
2001
บทคัดย่อ
A method for the determination of DON, NIV, 3 – acetylDON, fusarenon X, T – 2 toxin, HT-2 toxin, DAS and neosolaniol in wheat, based on capillary GC with flame ionisation detection (FID) has been developed and validated. The trichothecenes were extracted from the sample by acetonitrile/water (84/ 16, v/v). Two different Mycosep ® cleanup columns were used to purify the extract. The extract was evaporated to dryness and the trichothecenes were derivatised to trimethylsilyl ethers. The residue was dissolved in iso-octane and washed with water. The final extract was analysed for trichothecenes by GC with FID. The average recoveries ranged from 79% for NIV to 116% for DAS. The limit of quantification was 75 mg/Kg for each of the individual trichothecenes. A temporary tolerance limit of 500 mg/kg is in effect in the Netherlands for DON in cleaned wheat. A survey was carried out in the Netherlands in 1999 and 7/22 imported wheat samples exceeded this limit. Thirteen of the 22 wheat samples exceeded a proposed KON tolerance limit of 120 mg/kg for cleaned wheat.