บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Development of an HPTLC method for the determination of deoxynivalenol in cereal products.

OSTRY, V. and SKARKOVA, J.

JPC – Journal of Planar Chromatography – Modern TLC 13: 443 – 446.

2000

บทคัดย่อ

Development of an HPTLC method for the determination of deoxynivalenol in cereal products.

A method is described for the quantification of DON in cereals and cereal products (wheat flour and malt). The toxin was extracted with water and polyethylene glycol and the extracts were purified on immunoaffinity columns, then analysed by instrumental HPTLC on silica gel layers with cholroform – acetone – 2 – propanol (8 + 1 + 1) as mobile phase. The chromatogram was sprayed with a solution of aluminium chloride in ethanol to reveal the spots of DON and scanned in flurescence mode. Recovery was 84% in the range 0.2 – 0.67 mg/kg cereal products. The average RSD, was 6.9% and the limit of quantification was 0.2 mg/kg.