Suppression of spore germination and aflatoxin biosynthesis in Aspergillus parasiticus during and after exposure to high levels of phosphine.
ANTONACCI, L., SALVAT, A.E., FAIFER, G.C. and GODOY, H.M.
Mycopathologia 147:83-87.
1999
บทคัดย่อ
Agar cultures of toxigenic Aspergillus parasiticus were exposed to phosphine (PH3) at levels ranging from 0 to 2000 mg/kg. PH3 at concentrations of 400 mg/kg or higher completely arrested the growth of the fungus. When PH3 was vented and the agar plates were exposed to open air, 100% of the initial CFU developed into fully-grown colonies after PH3 levels below 300 mg/kg, but at higher PH3 concentrations only 50% of the colonies developed. The same strain of A. parasiticus was inoculated into high moisture corn. After exposure to PH3 at 500 mg/kg, both fungal growth and aflatoxin synthesis resumed shortly after elimination of the gas, but after exposure to PH3 levels of 1000 mg/kg and higher, there was reduced mycelial growth and almost complete absence of green pigmentation. In addition, aflatoxin synthesis was totally absent for the remainder of the experiment (20 days)