Biosynthesis of deoxynivalenol in spikelets of barley inoculated with macroconidia of Fusarium graminearum.
EVANS, C.K., XIE, W., DILL-MACKY, R. and MIROCHA, C.J.
Plant Disease 84: 654-660.
2000
บทคัดย่อ
The biosynthesis of DON and 15-acetyl-DON in barley spikelets inoculated with macroconidia of Fusarium graminearum (Group-II) was studied. Neither DON or 15-acetylDON was detected in macroconidia of 3 isolates. The samp isolates were pathogenic on barley cultivars Robust (moderately resistant) and produced DON and 15-acetylDON when grown in rice culture. The isolates were separately inoculated to a central spikelet on heads of cultivars Robust and Chevron. Both toxins were detected in spikelets 48 hr postinoculation (PI). Don increased dramatically after 72 hr and did not diminish thereafter. Accumulation of 15-acetylDON peaked at 72 to 120 hr and decreased by 240 hr PI. Differences of toxin accumulation at each sampling interval were significant when averaged over isolates and cultivars. Spikelets of 6 cultivars and lines were sampled at inoculation and 18, 36, 54, 72 and 90 hr PI. Don and 15-acetylDON were detected at 36 hr PI, but differences among the culivars and lines were not significant. Yield of DON in inoculated spikelets of 31 barley cultivars and lines at 72 hr PI ranged from 0.14 to 1.26 ug Per spikelet, and differences among the cultivars and lines were significant.