บทคัดย่องานวิจัย

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Determination of aflatoxins in grains and raw peanuts by a rapid procedure with fluorometric analysis.

MALONE, B.R., HUMPHREY, C.W., ROMER, T.R. and RICHARD, J.L.

Journal of AOAC International 83: 95-98.

2000

บทคัดย่อ

Determination of aflatoxins in grains and raw peanuts by a rapid procedure with fluorometric analysis.

A rapid, quantitative, inexpensive and efficient method was developed to determine aflatoxins in corn, corn meal, popcorn, rice, wheat, cottonseed and peanuts.  Samples are ground and extracted with methanol-water (80+20).  The extract is cleaned up by passage through a solid-hpase separatory column, derivatised with a bromine reagent, and fluorescence is immediately quantified with a fluorometer containing a broad wavelength pulsed xenon light source.  The method can quantify aflatoxin from 5-5000 ug/kg without dilution.  Correlation coefficients with LC for multiple analyses for corn, cottonseed and peanuts were 0.999,0.995 and 0.980, respectively.