Wheat seed colonized with atoxigenic Aspergillus flavus: Characterization and production of a biopesticide for aflatoxin control.
BOCK, C.H. and COTTy, P.J.
Biocontrol Science and Technology 9:529-543.
1999
บทคัดย่อ
Biocontrol of aflatoxin contamination in wheat using seed colonised with atoxigenic Aspergillus flavus has been used in commercial trials. Requirements for production of this colonised wheat seed are described and the spore yield of wheat was compared to other substrates. The study suggests that the most cost effective inoculum production would require colonisation of wheat (106 conidia/kg wheat sed) at 25% (w/w) moisture for 18 hr at 31ºC. The dry biopesticide had multi-year stability without refrigeration and withstood temperatures of 70ºfor 20 min. Sporulation of the product occurred winthin 3 days at 31ºC and 100% relative humidity with yields averaging 4.9 x 109 conidia/g by day 7.