Quantification of the mycotoxin patulin by a stable isotope dilution as say.
RYCHLIK, M. and SCHIEBERLE, P.
Journal of Agricultural and Food Chemistry 47: 3749-3755.
1999
บทคัดย่อ
Two stable isotope dilution assays for the quantification of patulin in foods were developed using [13C] – patulin as the internal standard. One method was performed by means of LC/MS in negative electrospray ionisation mode without derivatisation. The other used high resolution GC/high resolution MS (HRGC/HRMS) after trimethylsilylation of the patulin isotopomers. In comparison with previously reported methods based on HPLC with UV detection, HRGC/HRMS of the derivatised samples showed better repeatability, higher recovery rates and a 100 times lower detection limit (12 ng/L). In contrast, LC/MS showed a much lower performance as compared to HPLC/UV or HRGC/HRMS.