บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Alteration of difference domains in AFLR affects aflatoxin pathway metabolism in Aspergillus parasiticus transformants.

EHRLICH, K.C., MONTALBANO, B.G., BHATBAGAR, D. and CLEVELAND, T.E.

Fungal Genetics and Biology 23: 279-287.

1998

บทคัดย่อ

Alteration of difference domains in AFLR affects aflatoxin pathway metabolism in Aspergillus parasiticus transformants.

AFLR, a zinc binuclear cluster DNA binding protein, is required for activation of genes comprising the aflatoxin biosynthetic pathway in Aspergillus species. Transformation of A. parasiticus with plasmids containing the the intact aflR gene gave clones that produced 5 fold more aflatoxin pathway metabolites than did the untransformed strain. When a 13-bp region in the aflR promoter was deleted, including a portion of a palindromic site previously shown to bind recombinant AFLR, metabolite production was 40% that of transformants with intact aflR. This result provides further evidence that this site may be involved in the autoregulation of aflR.