Immunoassay of fumonisins by a surface plasmon resonance biosensor.
MULLETT, W., LAI, E.P.C. and YEUNG, J.M.
Analytical Biochemistry 258: 161 – 167.
1998
บทคัดย่อ
A surface plasmon resonance (SPR) immunosensor is reported for the determination of FB1 in spiked corn samples. Polyclonal antibodies produced against FB1 are adsorbed onto a thin gold film substrate, which is coupled to a glasses prism in the Kretschmann configuration. The output beam of a planar light emitting diode is focused through the prism to excite SPR at the surface of the gold film. When a sample containing FB1 is added to a cell on the outside of the gold film, the angular profile of reflected light intensity shifts. This changes the resonance angle and the reflected beam intensity at a selected angle, both of which are proportional of the antibody overlayer, a detection limit of 50mg/L is obtained for the direct assay with an analysis time under 10 min.