Determination of trichothecene mycotoxins in wheat by use of supercritical fluid extraction and high-performance liquid chromatography with diode array detection or gas chromatography with electron capture detection.
JOSEPHS, R.D., KRSKA, R., GRASSERBAUER, M. and BROEKAERT, J.A.C.
Journal of Chromatography A 795 : 297-304.
1998
บทคัดย่อ
The extraction behaviour of DON and some related type B trichothecenes from spiked and naturally contaminated wheat flour with modified supercritical CO2 has been investigated and optimised under several conditions. The extraction fluid was decompressed over a solid-phase trap and the amount of deposited analytes was determined by HPLC-diode array detection or GC-electron capture detection without any further cleanup. Recovery rates of around 90% were achieved for spiked wheat samples and 53% for naturally contaminated samples. The performance of the optimised supercritical fluid extraction method was compared with an already well established analytical method employing extraction on a rotary shaker in combination with Mycosep cleanup.