Cold hardiness and genetic relationships among Satsuma mandarin selections.
Cankui Zhang, Monte Nesbitt, Fenny Dane and Bob Ebel
Proceedings of 26th International Horticultural Congress. Volume of Abstract . Toronto, Canada, 11-17 August, 2002. Abstract S20-P-6. pp. 504-505.
2002
บทคัดย่อ
Satsuma mandarin [Citrus unshui (Marc.)], one of the most cold-tolerant citrus,is grown commercially along the northern coast of the Gulf of Mexico. Several recent Satsuma introductions from China are being tested for their degree of cold-hardiness and genetic variability. Cold hardiness is being evaluated using electrolyte leakage, callus formation of split of stem pieces and triphenyl tetrazolium chloride assays. Potted plant with roots thermally isolated are being exposed to –4, -6, -8, -10 and –12 0C in a temperature controlled freezer with a temperature decline rate of 2 0C/hr. Genetic variability is being determined from fingerprints using inter-simple sequence repeats (ISSR) and amplified fragment length polymorphisms (AFLP). Genetic distance between introduced accessions, known Satsuma cultivars, and mandarin and mandarin-like accessions will be calculated by PAUP analysis. Comparisons of cold hardiness and genetic variabillity will be discussed.