บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Peroxidase activity and tolerance/susceptibility to superficial scald development in apples

G. Paliyath, and D.P. Murr

Proceedings of 26th International Horticultural Congress. Volume of Abstract . Toronto, Canada, 11-17 August, 2002. Abstract S09-P-130. pp. 255.

2002

บทคัดย่อ

Peroxidase Activity and Tolerance/Susceptibility to Superficial Scald Development in Apples

Superficial scald is belived to result from a deficiency in the antioxidant status/function of apples. To alleviate health concerns associated with the present scald-prevention method that involves dipping or drenching apples with a solution of diphenylamine, potential biochemical prediction strategies were explored. Peroxidase (POX) is a very common enzyme that can be detected with ease and rapidity. A tissue printing method was developed to qualitatively determine the POX activity in the skin and hypodermal regions of apple fruits. Several common scald-susceptible apple varieties that included Mclntosh, Red Delicious, Cortland and ldared, and scald-resistant varieties such as ‘Empire’ , ‘ Gala’ and ‘Mutsu’ , were evaluated for POX reaction. In general, POX reaction resulted in the development of a near perfect ring of colour with varying intensities in the scald-resistant varieties, with ‘Empire’ showing the maximum intensity. By contrast, scald- susceptible varieties such as ‘Mclntosh’, ‘Cortland’ and ‘ldared’ showed very weak POX reaction. The results were quite variable in ‘red delicious’, with fruits from some orchards showing high intensity of POX reaction. Apples showing colour development. On this hypothetical basis, ‘Red Delicious’ and ‘Mclntosh’ apples from over 40 orchards and from two seasons were evaluated for their predicted future scald development soon after harvest, and actual scald development after storage in air at 2 C for four months. A positive correlation was observed between the POX reaction and resistance to superficial scald. Because of high variation, this technique could not be recommended as a reliable prediction strategy for superficial scald. Nevertheless, any technique that can increase the POX activity may potentially reduce the development of superficial scald.